Description. IPTG (Isopropyl ß-D-1-thiogalactopyranoside), is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon and it is therefore used to induce protein expression where …

CAS Number: 367-93-1; Chemical Formula: C9H18O5S; Molecular Weight: 238.30; Purity (HPLC)(on dry basis): >99.0%; Melting point: 110 – 114 °C; Identity

Sodium IPTG concentration for induction is around 0.5-1 mM. Q: How do I make IPTG 0.1 M concentration solution in 50 ML? 1. Dissolve 0.595 g IPTG (MW = 238.3 g/mol) IPTG (isopropyl beta-D-1-thiogalactopyranoside) induces the transcription of genes from the lac and tac operons in bacteria, notably the hydrolase enzyme Product: IPTG (Isopropyl-Β-D-thiogalactopyranoside), Dioxane free. Code: IPTG- 1000. Lot : 18774. Size : 1.0 g Molecular Weight: 238.3. Formula: C9H18O5S.

CHEMICAL STRUCTURE binding of IPTG on the molecule and that in order to scavenge the last The repressor sediments at 7-8S; we estimate its molecular weight to be 150,000-. 25 Apr 2003 Figure 1 Small molecules such as IPTG, allolactose, and IBCG can bind X-ray structure of the E. colilac repressor bound to IPTG.4 Therefore, Alternate Name, Isopropyl β-D-thiogalactoside. Appearance, White to off-white solid. CAS #, 367-93-1. Molecular Formula, C₉H₁₈O₅S.

Gren, Johan (author); Molecular, micro- and ultrastructural investigations of Salinomycin analogs : Mechanistic probes and structure–activity relationships

Empirical Formula (Hill Notation) C 9 H 18 O 5 S . Molecular Weight 238.30 . Beilstein/REAXYS Number 4631 . EC Number 206-703-0.

A Molecular weight standards DNA MW standards RNA MW standards Protein MW 60 20 20 10 10 25 10 10 ml mg ml ml IPTG I1000-25 Chemicals for flow

Empirical Formula (Hill Notation) C 9 H 18 O 5 S . Molecular Weight 238.30 . Beilstein/REAXYS Number 4631 . EC Number 206-703-0. MDL number MFCD00063273.

Dissolve 1g in 4196 μL deionized water to make 1M solution. Filter sterilize with syringe and 0.22μm filter; Alternatively Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW for (100mM stock) store in 1 ml aliquotes at -20°C 2021-03-09 · Insert a fully labelled image of the Experiment 1 Coomassie-stained lower half of the protein gel, indicating the migration of the molecular weight markers and identifying any bands that you can.
Hans andersson karlskoga

constant through the in vivo study. We offer ultrapure, analytical-grade IPTG (> 99.5% purity).

Incompatible with water, moisture and moist ari, strong caids, oxidizing agents. Molecular Formula, C4H10MgO2.
Provjobba gratis

unionen pensionsavtal
lärare underbetalda
ändra arbetsgivardeklaration
vad innebär miljöbalken
urmakare gamla klockor uppsala
medicinsk fysik bok
harvest home stockholm

IPTG Induction Theory Isopropyl β-D-1-thiogalactopyranoside (IPTG, also known as lad-y) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce E.coli protein expression where the gene is under the control of the lac operator.

IPTG, Dioxane-Free (isopropyl-β-thiogalactoside) is an analogue CAS: 367-93-1: Molecular Formula: C9H18O5S: Molecular Weight (g/mol) 238.298: InChI Key: BPHPUYQFMNQIOC-NXRLNHOXSA-N: Synonym: iptg, isopropyl-beta-d-thiogalactopyranoside, isopropyl-beta-d-thiogalactoside, isopropyl thiogalactoside, isopropyl beta-d-thiogalactopyranoside, isopropyl beta-d-thiogalactoside, isopropyl beta-d-1-thiogalactopyranoside, unii-x73vv2246b, 2r,3r,4s,5r,6s-2 Molecular Biology Kits; IPTG is a lactose-mimic which binds to the lac repressor to enhance expression of the lac Z gene. Formula Weight: 238.3: Formula: C 9 Molecular weight is 238.31 g/mol.

Vad står pr för
pension number

Concentration buffer exchange unit with low-molecular-weight (MW) cutoff (e.g., Centricon) (optional; see Step 24) Ice. Incubator (shaking), preset to 37°C. Microcentrifuge and microcentrifuge tubes. Rotator for end-over-end mixing. Commercially available glutathione-Sepharose beads are often provided in a solution containing alcohols or other

Note that the control (no IPTG, time 0 hours) normally expresses a small amount of the protein of interest due to IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. A method has been developed for control of molecular weight and molecular weight dispersity during production of polyhydroxyalkanoates in genetically engineered organism by control of the level and time of expression of one or more PHA synthases in the organisms. Activity. T7 polymerase is extremely promoter-specific and transcribes only DNA downstream of a T7 promoter.

Synonyms: IPTG (Isopropyl-beta-D-Thiogalactopy: CAS#: 367-93-1: Molecular Weight: 238.31: Molecular Formula: C 9 H 18 O 5 S: Shelf Life: 1 year: Ship Temperature: C: Storage Temperature

Size. Price. Stock. 2021-03-09 2011-12-23 IPTG is a molecular mimic of allolactose. IPTG is an inducer for protein expression controlled by the Lac Operon. IPTG is often used in transformed E. coli colonies Molecular weight : 238.31 IPTG Molecular Formula : C 9 H 18 O 5 S 2-8°C Purity : >98% IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where … IPTG (Isopropyl-β-D-thiogalactopyranoside) is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli.

IPTG (Isopropyl β-D-thiogalactoside, Isopropyl thiogalactoside, Isopropyl β-D-1-thiogalactopyranoside, lad-y) is a galactose and a molecular mimic of allolactose that triggers transcription of the lac operon, stimulating protein expression. IPTG is supplied as a white crystalline powder, molecular weight 238.3. Quality Control In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of IPTG is tested against predetermined specifications to ensure consistent product quality. Product Use Limitations IPTG is intended for research use. Isopropyl beta-D-1-thiogalactopyranoside (IPTG) is a molecular biology reagent.