One of the most widespread Lactobacillus species used in food technology is Lactobacillus plantarum, which has a homofermentative metabolism, a high acid tolerance and is, like most LAB, a Generally Regarded As Safe (GRAS) organism. Experiments with defined media have shown that Lact. plantarum NC8 needs arginine, leucine, isoleucine,

2019-04-02

Buscoi. Lactobacillus plantarum (Orla-Jensen) Bergey et al. (ATCC® 8014™). Each NCIB culture from a different depositorAccumulation of biotinD-biotin conversion   Jun 17, 2019 Hansen has developed a new freeze dried, direct inoculation culture of Lactobacillus plantarum. Chr. Hansen's Harvest LB-1 is the first pure  A single strain of L. plantarum that performs well in kettle souring/sour worting where fast and clean lactic acidity is desired.

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In this study we examine the expression of heterologous 2014-08-22 L. plantarum strain NC8 (CCUG 61730) and the Escherichia coli- Lactobacillus shuttle vector pSIP409 were kindly provided by Professor A. Kolandaswamy (Madurai Kamaraj University, India). The pValac-GFP plasmid was kindly provided by Anderson Miyoshi [ 18 ]. Plantaricin NC8 (PLNC8), a coculture-inducible two-peptide bacteriocin from Lactobacillus plantarum NC8, has recently been purified and genetically characterized. Analysis of an 8.1-kb NC8 DNA region downstream of the PLNC8 operon revealed the presence of at least four operons involved in bacteriocin production, showing high homology to the plantaricin cluster in L. plantarum C11. 2020-02-11 2021-04-17 Plantaricin NC8 from Lactobacillus plantarum causes cell membrane disruption to Micrococcus luteus without targeting lipid II Author: Jiang Han Tang Xuan Zhou Qingqing Zou Jiong Li Ping Breukink Eefjan Gu Qing Journal: Applied Microbiology and Biotechnology Issue Date: 2018 Lactic acid bacteria (LABs) are being used as a probiotic very often for various enteric problems. Many genetically modified LABs are created by different workers for various novel applications.

2020-02-11 · L. plantarum NC8 was cultured in De Man, Rogosa and Sharpe (MRS) medium at 30 °C without agitation. L. plantarum NC8-competent cells were prepared as previously described . The L. plantarum NC8/Δalr strain was cultured in MRS medium with 200 μg/mL d-ala (Sigma, USA).

Fermentis, SafSour™ LP 652, L. plantarum appears to contribute more to alleviating IBS than other probiotic strains, but more research is needed. L. reuteri.

Lactobacillus plantarum is a versatile bacterium found in a variety of ecological niches, ranging from vegetable and plant fermentations to the human gastrointestinal tract. L. plantarum cells are rods with rounded ends, straight, generally 0.9–1.2 μm wide and 3–8 μm long, occurring singly, in pairs or in short chains (Figure 43.1).

We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many laboratories worldwide. 2004-03-01 Purification and Genetic Characterization of Plantaricin NC8, a Novel Coculture-Inducible Two-Peptide Bacteriocin from Lactobacillus plantarum NC8. Applied and Environmental Microbiology, 2003. A. Alcocer Maldonado. Download PDF. Download Full PDF Package. This paper. A … Plantaricin NC8 alpha peptide. Gene.

2004-03-01 However, little is known about the role of IL-17B in the control of viral infections. In this study, a recombinant Lactobacillus plantarum, designated as NC8-ChIL17B, was constructed to express the chicken IL-17B (ChIL-17B) gene.
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It encodes a polypeptide of 474 residues having 55% identities with l- AIs from Bacillus stearothermophilus US100 and Thermus sp. IM6501. However, little is known about the role of IL-17B in the control of viral infections. In this study, a recombinant Lactobacillus plantarum, designated as NC8-ChIL17B, was constructed to express the chicken IL-17B (ChIL-17B) gene. The recombinant ChIL17B (rChIL17B) protein was about 14 kDa and was anchored to the surface of NC8 cells.

Plantaricin NC8, a two-peptide non-lantibiotic class IIb bacteriocin composed of PLNC8α and PLNC8β and derived from Lactobacillus plantarum ZJ316, has been shown to be highly potent against a range of bacteria and fungi. Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract.
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3 Lactobacillus plantarum NC8 was shown to produce plantaricin NC8 (PLNC8), a recently 4 purified and genetically characterized inducible class IIb bacteriocin, only when it was co-5 cultured with other Gram-positive bacteria. Among 82 strains belonging to the genera

Transformation of Lb. sakei Lb790, Lb. plantarum NC8 and Lb. casei CECT475 with plasmids pSIP409UAI, pSIP411UAI and pMGUAI yielded recombinant Lactobacillus spp.-derived strains which were further checked by bacteriocinogenicity tests, PCR and sequencing of the inserts. To construct the recombinant Lactobacillus plantarum NC8for expression of HA-DCpep fusion gene and to detect the immunogenicity of fusion protein,the HA-DCpep gene was amplified by PCR and cloned into pGEM-T vector.The target gene was cloned into Escherichia coli-Lactobacillus shuttle expression vector pSIP409after sequencing.The recombinant plasmid was electroporated into L.plantarum NC8,and Lactic acid bacteria (LABs) are being used as a probiotic very often for various enteric problems.

2004-03-01

Analysis of an 8.1-kb NC8 DNA region downstream of the PLNC8 operon revealed the presence of at least four operons involved in bacteriocin production, showing high homology to the plantaricin cluster in L. plantarum C11. Lactobacillus plantarum NC8 Host strain, silage isolate Plasmids pLPV111 Source of 256rep, pUC(pGEM)ori and ermL pKRV3 Source of sapIP*KR, sapKR and T saiA pGEM®-7Zf(+) Source of MCS Promega, USA pSAK17B Source of P sapA pMLS114 Source of P sppA, sppIP*KR and sppKR pNZ9531 Source of P nisRK and nisRK Many genetically modified LABs are created by different workers for various novel applications. In this study we examine the expression of heterologous oxalate decarboxylase (oxdc) in Lactobacillus plantarum NC8. Generally, this enzyme is not present in Lactobacillus spp. Oxdc gene from Bacillus Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract.

In addition, recombinant L. plantarum NC8 strains expressing the M2e or HA2 antigen constructed by Yang et al.